日韩中文日韩欧美在线观看,亚洲人成网站999久久久综合,精品无码三级在线观看视频,亚洲一区爱区精品无码,亚洲第一AV无码专区,亚洲s久久久久一区二区,国产免费丝袜调教视频,国产在线视频一区二区三区,亚洲免费毛片,国产三级裸体视频无码

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > 行業(yè)資訊 > K-12 MG1655大腸桿菌克隆菌株

K-12 MG1655大腸桿菌克隆菌株

 K-12 MG1655大腸桿菌克隆菌株

貨號                          名稱                          規(guī)格 儲存
BMZ066590   K-12 MG1655 Strain 100μl -20℃
 
明舟生物的各批次質(zhì)粒菌株發(fā)貨前均經(jīng)過嚴(yán)格的多重驗(yàn)證,如存在質(zhì)量問題,請?jiān)谑盏疆a(chǎn)品三個(gè)月內(nèi)通知我司。收到甘油菌后,請及時(shí)在固體平板上劃線培養(yǎng),挑取單菌落接種到液體培養(yǎng)基中,震蕩培養(yǎng)后使用并保存甘油菌。如需獲取菌株其他詳細(xì)信息請登錄我司官網(wǎng)聯(lián)系查詢。
 
基因型:
F- lambda- ilvG- rfb-50 rph-1(NC_000913)
 
基本信息:
抗性:無
培養(yǎng)基:LB
菌株類別:大腸桿菌
培養(yǎng)條件:37℃,有氧,LB
質(zhì)粒轉(zhuǎn)化:42℃熱激
保存方式:20%甘油,-20℃
基本應(yīng)用:用于基因克隆
 
菌株簡介:
   K-12 MG1655是一株K系列的大腸桿菌,經(jīng)檢測無Amp和Kan抗性,可用LB培養(yǎng)基,37℃培養(yǎng),其代謝類型是異養(yǎng)兼性厭氧型,可用于代謝研究、基因編輯等實(shí)驗(yàn)。
The original K-12 wild-type strain from the Stanford collection contained both the F plasmid and phage lambda. MG1655 was constructed by curing that strain using acridine orange and UV.This strain was sequenced by the Blattner laboratory because it approximates wild-type E. coli and "has been maintained as a laboratory strain with minimal genetic manipulation, having only been cured of the temperate bacteriophage lambda and F plasmid by means of ultraviolet light and acridine orange, respectively." (Blattner, et al. 1997). The mutations listed in the genotype are present in most K-12 strains and were probably acquired early in the history of the laboratory strain. A frameshift at the end of rph results in decreased pyrE expression and a mild pyrimidine starvation, such that the strain grows 10 to 15% more slowly in pyrimidine-free medium than in medium containing uracil (Jensen 1993). The ilvG- mutation is a frameshift that knocks out acetohydroxy acid synthase II (Lawther, et al. 1982). The rfb-50 mutation is an IS5 insertion that results in the absence of O-antigen synthesis (Liu and Reeves 1994).
MG1655 was derived and named by Mark Guyer from strain W1485, which was derived in Joshua Lederberg's lab from a stab-culture descendant of the original K-12 isolate. This original E. coli strain K-12 was obtained from a stool sample of a diphtheria patient in Palo Alto, CA in 1922 (Bachmann, B., pp. 2460-2488 in Neidhardt et al.1996, Escherichia coli and Salmonella: Cellular and Molecular Biology, ASM Press).MG1655 grows on LB and M9 minimal medium (+ Glucose + 1ug/ml thiamine).
 
注意事項(xiàng):
1、為了您的安全和健康,請穿實(shí)驗(yàn)服并戴一次性手套操作。本產(chǎn)品僅可用于實(shí)驗(yàn)室研究,不能用于動物,人體以及作為食品添加劑等用途。
2、使用甘油菌時(shí)可不完全融解,在甘油菌表面蘸取少量涂布固體瓊脂平板即可,也可完全融解后使用,但隨著凍融次數(shù)的增加,菌株的活力會逐漸下降。
 
广灵县| 洛隆县| 商河县| 孝感市| 桂林市| 神木县| 连云港市| 黄梅县| 清远市| 宜黄县| 彭泽县| 雷山县| 克山县| 大厂| 梧州市| 三门县| 崇州市| 仪陇县| 富民县| 永仁县| 亳州市| 渭源县| 东兰县| 蕉岭县| 右玉县| 旺苍县| 德昌县| 贵德县| 梓潼县| 梁山县| 汾西县| 祁门县| 安龙县| 关岭| 英山县| 来凤县| 沙田区| 阿克| 永城市| 神农架林区| 兴文县|