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13762 MAT B III

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編號(hào):B162378

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產(chǎn)品名稱	13762 MAT B III
商品貨號(hào)	B162378
Organism	Rattus norvegicus, rat
Tissue	mammary gland
Product Format	frozen
Morphology	epithelial
Culture Properties	suspension or loosely adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	adenocarcinoma
Strain	Fischer 344 (F344)
Storage Conditions	liquid nitrogen vapor phase
Derivation	The 13762 MAT B III cell line was established at the EG&G Mason Research Institute from a transplantable rat ascites tumor derived from the 13762 solid mammary adenocarcinoma.
Comments	Prior to its deposit, the cell line was tested and found negative for the following viruses: Pneumonia virus of mice (PVM), reovirus type 3 (Reo3), K virus, polyomavirus, Sendai virus, GDVII (mouse poliovirus), Kilham rat virus (KRV), minute virus of mice (MVM), SV5, H-1 virus and ectromelia virus. The line grows as small clusters of rounded cells lightly attached to the substrate, or as a static suspension.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 x 10⁵ viable cells/mL and maintain at 1 x 10⁵ and 1 x 10⁶ cells/mL. Medium Renewal: Every 2 to 3 days
Cryopreservation	Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase
Culture Conditions	Temperature: 37°C
Name of Depositor	AE Bogden
Deposited As	Rattus sp.
References	Xing RH, et al. Prevention of breast cancer growth, invasion, and metastasis by antiestrogen tamoxifen alone or in combination with urokinase inhibitor B-428. Cancer Res. 57: 3585-3593, 1997. PubMed: 9270032

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