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Mumps rubulavirus
Mumps rubulavirus
規(guī)格:
貨期:
編號(hào):B162814
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Mumps rubulavirus
商品貨號(hào) B162814
Classification Paramyxoviridae, Rubulavirus
Agent Mumps rubulavirus
Strain MuV/Iowa.US/2006 (Genotype G)
Common Name Mumps virus
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL per vial
Storage Conditions -70°C or colder
Genome Sequenced Strain

Mumps virus strain MuV-IA, complete genome

Images VR-1899 Images
Effect on Host
CPE, cell fusion and sloughing
Recommended Host
Vero E6 cells (ATCC® CRL-1586™)
Growth Conditions

Temperature: 37°C

Recommendations for Infection: Plate cells 18-24 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.001-0.01). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere. End adsorption by adding virus growth medium.

Incubation: 3-7 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.

Name of Depositor P Rota, CDC
Source Isolated by oral swab from a human in Iowa, USA, 2006
References

Boddicker JD, et al. Real-time reverse transcription-PCR assay for detection of mumps virus RNA in clinical specimens.  J Clin Microbiol. 45(9): 2902-2908, 2007.  PubMed: 17652480

Xu P, et al. Rescue of wild-type mumps virus from a strain associated with recent outbreaks helps to define the role of the SH ORF in the pathogenesis of mumps virus.  Virology 417(1): 126-136, 2011.  PubMed: 21676427

Cross References

Nucleotide (GenBank) : JN012242.1 Mumps virus strain MuV-IA, complete genome

Nucleotide (GenBank) : DQ661745.1 Mumps virus strain MUM/Iowa.US/2006 small hydrophobic protein mRNA, complete cds

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沈經(jīng)理 19548299266 2662369050
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