| 產(chǎn)品名稱 |
H-1 parvovirus |
| 商品貨號(hào) |
B162882 |
| Classification |
Parvoviridae, Parvovirinae, Parvovirus |
| Deposited As |
H-1 virus |
| Agent |
H-1 parvovirus |
| Strain |
Toolan |
| Applications |
Produces deformity or death in newborn hamsters and neutralizing antibodies in humans, monkeys, hamsters and rats. |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Product Format |
frozen 1.0 mL per vial |
| Storage Conditions |
?-70°C or colder |
| Genome Sequenced Strain |
Parvovirus h-1, complete genome
|
| Comments |
Plaque purified on Salk MkHe. Hemagglutinates a variety of RBC, particularly guinea pig, hamster and human, and also has a CF antigen. Produces deformity or death in newborn hamsters and neutralizing antibodies in humans, monkeys, hamsters and rats.
|
| Effect on Host |
Cytopathic effects (cell death) in tissue culture; death or deformity of newborn hamster depending on virus concentration |
| Recommended Host |
Chang L cells (ATCC® CCL-13™)
|
| Growth Conditions |
Temperature: 37°C
Recommendations for Infection: Plant cells 24-48 hours in advance and infect when cultures are about 70% confluent. Remove medium and inoculate with a small volume of virus diluted to provide a MOI of about 0.1. Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: Incubate infected culture for 5 days at 37°C temperature in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer. |
| Name of Depositor |
HW Toolan |
| Source |
Human embryo lung (4 months operative abortion) from mother with metastatic breast cancer, who had an HAI and neutralizing titer of 1:640 to H-1 virus. |
| References |
Toolan H, Ledinko N. Growth and cytopathogenicity of H-viruses in human and simian cell cultures. Nature 208: 812-813, 1965. Pubmed: 4958078
Karasaki S. Size and ultrastructure of the H-viruses as determined with the use of specific antibodies. J Ultrastruct Res 16(1): 109-122, 1966. Pubmed: 4959542
Proc. Am. Assoc. Cancer Res. 5: 64, 1964.
Rhodes SL, Paradiso PR. Parvovirus genome: nucleotide sequence of H-1 and mapping of its genes by hybrid-arrested translation. J Virol 45(1): 173-184, 1983.
|
| Cross References |
Nucleotide (GenBank) :
X01457.1
Parvovirus h-1, complete genome
|
