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Feline infectious peritonitis virus
Feline infectious peritonitis virus
規(guī)格:
貨期:
編號(hào):B163028
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 Feline infectious peritonitis virus
商品貨號(hào) B163028
Classification Coronaviridae, Alphacoronavirus, Alphacoronavirus 1
Agent Feline infectious peritonitis virus
Strain DF2
Biosafety Level 2

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen 1.0 mL
Storage Conditions -70°C or colder
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Images ATCC VR-2004 images
Comments Cross reacts with canine coronavirus, TGE and human 229B.
Effect on Host
CPE, Cell rounding and degeneration
Recommended Host
CRFK cells (ATCC® CCL-94™)
Growth Conditions

Temperature: 37°C

Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm2). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.

Incubation: 3-5 days at 37°C in a humidified 5% CO2 atmosphere, until CPE is progressed through 80% of the monolayer.

Name of Depositor EV Davis
U.S. Patent
U.S. Patent Number
Special Collection ATCC
Source Spleen of cat with typical FIP disease
References

Evermann JF, et al. Characterization of a feline infectious peritonitis virus isolate. Vet. Pathol. 18: 256-265, 1981. PubMed: 7467085

McKeirnan AJ et al. Comparative properties of feline coronaviruses in vitro. Can. J. Vet. Res. 51(2): 212-216, 1987. PubMed: 1255305

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
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