Chlamydophila pneumoniae (Grayston et al.) Everett et al.
商品貨號(hào)
B163418
Classification
Chlamydiaceae, Chlamydophila
Deposited As
Chlamydia pneumoniae Grayston et al.
Agent
Chlamydophila pneumoniae (Grayston et al.) Everett et al.
Strain
J-21
Biosafety Level
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Comments
Isolate from patient in Japan
This preparation has tested positive for Mycoplasma contamination
FBS used to culture Chlamydia must be prescreened to verify that the serum does not contain antibodies to Chlamydia or other factors that would interfere with growth.
Effect on Host
Intracellular inclusion bodies visualized by fluorescent staining with genus or species specific conjugated antibodies
Recommended Host
HEp-2 cells (ATCC CCL-23)
Growth Conditions
Temperature: 35°C Recommendations for Infection: For best results cells should be 24 to 48 hours old and 90-100% confluent. Incubation: 3 days, a 5% CO2 in air atmosphere is recommended
Name of Depositor
N Tsumura
Source
Throat culture of 12-year-old boy in Japan with pneumonia
References
Tsumura N. Chlamydia pneumoniae lower respiratory tract infections in Japanese children. Abstract: Annual Meeting ICAAC. San Francisco: ; 1995.