Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody is specific for the non-reducing terminal N-acetyllactosamine and does not react with glycolipids having a type I chain, a ganglio series structure or a sialosyl or fucosyl substitution at the N-acetyllactosamine residue.
Tested and found negative for ectromelia virus (mousepox).
Derivation
Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against glycosphingolipid
Cellular Products
immunoglobulin; monoclonal antibody; against glycosphingolipid
Comments
Animals were immunized with lacto-N-nor-hexaosylceramide non-covalently adsorbed to naked Salmonella minnesota.
Spleen cells were fused with NS-1 myeloma cells.
The antibody is specific for the non-reducing terminal N-acetyllactosamine and does not react with glycolipids having a type I chain, a ganglio series structure or a sialosyl or fucosyl substitution at the N-acetyllactosamine residue.
The antibody agglutinates all adult human erythrocytes but not umbilical cord blood erythrocytes.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
RPMI 1640 medium, 85%; fetal bovine serum, 15%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
Isotype
IgM
Name of Depositor
WW Young
Deposited As
mouse (B cell); mouse (myeloma)
References
Young WW Jr., et al. Two monoclonal anticarbohydrate antibodies directed to glycosphingolipids with a lacto-n-glycosyl type II chain. J. Biol. Chem. 256: 10967-10972, 1981. PubMed: 7287743
