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1B2A3
1B2A3
規(guī)格:
貨期:
編號:B163615
品牌:Mingzhoubio

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產品名稱 1B2A3
商品貨號 B163615
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody differentiates toxin producing strains of P. multocida from other isolates, and can be used to identify toxigenic strains of P. multocida.
The 1B2A3 hybridoma was established by Kevin W. Ruby and Bill Knudtson in 1990.
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with a toxoid preparation purified from the NOR-1 strain of P. multocida (the toxoid preparation was a gift from J.C. Frantz).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The 1B2A3 hybridoma was established by Kevin W. Ruby and Bill Knudtson in 1990.
Genes Expressed
immunoglobulin; monoclonal antibody; against Pasturella multocida type D dermonecrotic toxin
Cellular Products
immunoglobulin; monoclonal antibody; against Pasturella multocida type D dermonecrotic toxin
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with a toxoid preparation purified from the NOR-1 strain of P. multocida (the toxoid preparation was a gift from J.C. Frantz).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody differentiates toxin producing strains of P. multocida from other isolates, and can be used to identify toxigenic strains of P. multocida.
The 1B2A3 hybridoma was established by Kevin W. Ruby and Bill Knudtson in 1990.
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL.  Maintain cultures at a cell concentration between 2 x 105 and 1 x 106 cells/mL.
Medium Renewal: Two to three times weekly
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG1
Name of Depositor LA Elsken
Originators: KW Ruby,W Knudtson
Deposited As mouse (B cell); mouse (myeloma)
Year of Origin 1990
References

Martineau-Doize B, et al. Effects of purified Pasteurella multocida dermonecrotoxin on cartilage and bone of the nasal ventral conchae of the piglet. Anat. Rec. 228: 237-246, 1990. PubMed: 2260779

Magyar T, Rimler RB. Detection and enumeration of toxin-producing Pasteurella multocida with a colony-blot assay. J. Clin. Microbiol. 29: 1328-1332, 1991. PubMed: 1885729

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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