| 產(chǎn)品名稱 |
4F2C13 |
| 商品貨號(hào) |
B163770 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Leukemia |
| Applications |
This line is derived from the same fusion used to produce ATCC HB-2 and ATCC HB-21. The antibody reacts with peripheral blood monocytes and to a lesser extent peripheral blood T cells. The antibody fixes complement and binds to Protein A. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with P3X63Ag8 myeloma cells. This line is derived from the same fusion used to produce ATCC HB-2 and ATCC HB-21. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human monocyte |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human monocyte |
| Tumorigenic |
Yes |
| Effects |
Yes, in pristane primed BALB/c mice |
| Comments |
Animals were immunized with HSB-2 cells (ATCC CCL-120.1, an acute lymphocytic T leukemia cell line). Spleen cells were fused with P3X63Ag8 myeloma cells. This line is derived from the same fusion used to produce ATCC HB-2 and ATCC HB-21. The antibody reacts with peripheral blood monocytes and to a lesser extent peripheral blood T cells. The antibody fixes complement and binds to Protein A. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Freeze medium: complete growth medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Isotype |
IgG2a; kappa light chain |
| Name of Depositor |
A Fauci, BF Haynes |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Eisenbarth GS, et al. Production of monoclonal antibodies reacting with peripheral blood mononuclear cell surface differentiation antigens. J. Immunol. 124: 1237-1244, 1980. PubMed: 6153676
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