日韩中文日韩欧美在线观看,亚洲人成网站999久久久综合,精品无码三级在线观看视频,亚洲一区爱区精品无码,亚洲第一AV无码专区,亚洲s久久久久一区二区,国产免费丝袜调教视频,国产在线视频一区二区三区,亚洲免费毛片,国产三级裸体视频无码

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > 4H1-A7
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
4H1-A7
4H1-A7
規(guī)格:
貨期:
編號:B163771
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 4H1-A7
商品貨號 B163771
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody binds to and neutralizes CAV-2 and is type specific.
Tested and found negative for ectromelia virus (mousepox).
Storage Conditions liquid nitrogen vapor phase
Derivation
Animals were immunized with purified CAV-2 (Manhattan strain).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against canine adenovirus type 2 (CAV-2)
Cellular Products
immunoglobulin; monoclonal antibody; against canine adenovirus type 2 (CAV-2)
Tumorigenic Yes
Effects
Yes, in BALB/c mice
Comments
Animals were immunized with purified CAV-2 (Manhattan strain).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody binds to and neutralizes CAV-2 and is type specific.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%
Subculturing

Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 105 viable cells/mL.  Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL.

Medium Renewal: Two to three times weekly
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG2a
Name of Depositor CA Whetstone
Deposited As mouse (B cell); mouse (myeloma)
References

Whetstone CA. Monoclonal antibodies to canine adenoviruses 1 and 2 that are type- specific by virus neutralization and immunofluorescence. Vet. Microbiol. 16: 1-8, 1988. PubMed: 3281371

Whetstone CA. Should the criteria for species distinction in adenoviruses be reconsidered? Evidence from canine adenoviruses 1 and 2. Intervirology 23: 116-120, 1985. PubMed: 3884549

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
于經(jīng)理 18067160830 2088210172
沈經(jīng)理 19548299266 2662369050
李經(jīng)理 13626845108 972239479
渝中区| 社旗县| 金湖县| 鄂伦春自治旗| 侯马市| 普兰店市| 宜宾县| 克拉玛依市| 姜堰市| 聊城市| 绍兴县| 福泉市| 永泰县| 吴旗县| 铜山县| 抚顺市| 河间市| 仙居县| 华安县| 衡阳县| 五河县| 县级市| 蚌埠市| 桦川县| 曲松县| 五台县| 大冶市| 棋牌| 黄大仙区| 永泰县| 北辰区| 峨眉山市| 获嘉县| 芷江| 上杭县| 新田县| 田东县| 乐东| 明水县| 元阳县| 织金县|