產(chǎn)品名稱 |
pCMM17.1 |
商品貨號(hào) |
B236384 |
Designations |
pCMM17.1 |
Species |
Homo sapiens, human |
Depositors |
RL White, Y Nakamura |
Applications |
Codominant segregation of the MspI RFLP was observed in 48 3-generation families. |
Vector |
Construct size (kb): 5.099999904632568 |
Insert |
DNA: genomic Insert lengths(kb): 2.299999952316284 Gene product: DNA Segment, single copy [D10S16] Alleles: A1, A1, A2, A2, M1, M1, M2, M2 |
Insert Size (kb) |
2.300 |
Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
Shipping Information |
Distributed: freeze-dried |
Comments |
Restriction digests of the clone give the following sizes (kb): EcoRI/HindIII--2.7, 2.3; PstI/BamHI--2.8, 1.9, 0.43; HindIII--5.0; BamHI--5.0; EcoRI--5.0. An EcoRI/HindIII double digest shows an insert fragment size of 2.4 kb. Codominant segregation of the MspI RFLP was observed in 48 3-generation families. Enzyme(s) not detecting polymorphism: TaqI, RsaI, PstI, BglII, PvuII. |
References |
Lathrop M, et al. A primary genetic map of markers for human chromosome 10. Genomics 2: 157-164, 1988. PubMed: 2900809
White RL, et al. The CEPH consortium primary linkage map of human chromosome 10. Genomics 6: 393-412, 1990. PubMed: 1970325
Martin C, et al. Isolation and mapping of a polymorphic DNA sequence (pCMM17.1) on chromosome 10 [D10S16]. Nucleic Acids Res. 16: 3121, 1988. PubMed: 2897114
Yusuke Nakamura, personal communication
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