Comments |
Restriction digests of the clone give the following sizes (kb): SalI--5.6, PstI--2.77, 1.5, 1.39, 1.0; HindIII--5.6; EcoRI--2.6 doublet; BamHI--4.8, 0.53. The PstI polymorphism demonstrates codominant segregation in more than 50 families. RFLPs observed under normal stringency; washes at 0.1X SSC, 0.1% SDS, 65 C. Enzyme(s) not detecting polymorphism: MspI, TaqI. |
References |
Nakamura Y, et al. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science 235: 1616-1622, 1987. PubMed: 3029872
Nakamura Y, et al. Isolation and mapping of a polymorphic DNA sequence pYNM3 on chromosome 8 (D8S38). Nucleic Acids Res. 15: 10080, 1987. PubMed: 2892174
Peter J O'Connell, personal communication
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