| 產(chǎn)品名稱 |
Waddlia chondrophila Everett et al. |
| 商品貨號 |
B236937 |
| Classification |
Waddliaceae |
| Agent |
Waddlia chondrophila Everett et al. |
| Strain |
WSU 86-1044 |
| Applications |
Cross-reactivity was observed with Cowdria ruminantium (Kiswani isolate). Reference antisera against Coxiella, Wolbachia, Chlamydia, and Anaplasma spp. Identification is recommended by 16S rRNA sequence, GenBank accession number AF042496. |
| Biosafety Level |
2
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Isolation |
pooled liver/lung homogenate from the aborted fetus of a 19-month-old primigravid Holstein cow during month 3 of gestation |
| Product Format |
frozen |
| Storage Conditions |
-70°C or colder |
| Genome Sequenced Strain |
Yes
|
| Comments |
Reference antisera against Coxiella, Wolbachia, Chlamydia, and Anaplasma spp. and 14 members of the genus Rickettsia all failed to react significantly. Cross-reactivity was observed with Cowdria ruminantium (Kiswani isolate). Identification is recommended by 16S rRNA sequence, GenBank accession number AF042496. MEM with 10% serum |
| Effect on Host |
Dense forms enter cytoplasm and reticulated forms develop, then change to dense forms and are released from the cell Inclusions visualized by light microscopy as dense forms.? Some cell death due to lysis.? Cells become rounded and dense. |
| Recommended Host |
bovine turbinate (BT) cells (ATCC CRL-1390) BT cells (ATCC CRL-1390); P388D1 mouse macrophage cells (ATCC CCL-46) |
| Growth Conditions |
Incubation: 3 days at 35°C in BT cells (, 5% CO2 |
| Name of Depositor |
TF McElwain |
| Special Collection |
NCRR Contract |
| Source |
pooled liver/lung homogenate from the aborted fetus of a 19-month-old primigravid Holstein cow during month 3 of gestation |
| References |
Dilbeck PM, et al. Isolation of a previously undescribed rickettsia from an aborted bovine fetus. J. Clin. Microbiol. 28: 814-816, 1990. PubMed: 2185269
Kocan KM, et al. Development of a rickettsia isolated from an aborted bovine fetus. J. Bacteriol. 172: 5949-5955, 1990. PubMed: 2211519
Rurangirwa FR, et al. Analysis of the 16S rRNA gene of micro-organism WSU 86-1044 from an aborted bovine foetus reveals that it is a member of the order Chlamydiales: proposal of Waddliaceae fam. nov., Waddlia chondrophila gen. nov., sp. nov.. Int. J. Syst. Evol. Microbiol. 49: 577-581, 1999. PubMed: 10319478
type strain
Terry F McElwain, personal communication
|
| Cross References |
Nucleotide (GenBank) :
AF042496
16S ribosomal RNA and 23S ribosomal RNA, complete sequence
|
