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<em>Saccharomyces cerevisiae</em> Meyen ex E.C. Hansen
<em>Saccharomyces cerevisiae</em> Meyen ex E.C. Hansen
規(guī)格:
貨期:
編號:B244213
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
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甘油
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產(chǎn)品名稱 Saccharomyces cerevisiae Meyen ex E.C. Hansen
商品貨號 B244213
Deposited As Saccharomyces cerevisiae Hansen, teleomorph
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
Strain Designations YPH98
Alternate State Candida robusta Diddens et Lodder
Application
Host strains for transformation, P. Hieter strains (BA1)
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Type Strain no
Genotype MATa ura3-52 lys2-801(amber mutation) ade2-101(ochre mutation) leu2-delta1 trp1-delta1 RefSikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436, (The trp1-delta1 gene does not retain homology to the TRP1 selectable marker while the trp1-delta63 gene does. trp1-delta1 also deletes the UAS elements required for expression of the adjacent GAL3 gene, whereas trp1-delta63 does not. trp1-delta1 strains are therefore gal3 whereas trp1-delta63 strains remain Gal+ .)
Preceptrol&reg; no
Mating Type a
Ploidy haploid
Comments
Host strains for transformation, P. Hieter strains (BA1)
Medium ATCC® Medium 1069: YPAD medium
Growth Conditions
Temperature: 25.0°C
Atmosphere: Typical aerobic
Name of Depositor YGSC
Special Collection Yeast Genetic Stock Center
Chain of Custody
ATCC <<--YGSC<<--R. Sikorski and P. Hieter
References

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436

The trp1-delta1 gene does not retain homology to the TRP1 selectable marker while the trp1-delta63 gene does. trp1-delta1 also deletes the UAS elements required for expression of the adjacent GAL3 gene, whereas trp1-delta63 does not. trp1-delta1 strains are therefore gal3 whereas trp1-delta63 strains remain Gal+ .

Sikorski RS, Hieter P. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27, 1989. PubMed: 2659436

The trp1-delta1 gene does not retain homology to the TRP1 selectable marker while the trp1-delta63 gene does. trp1-delta1 also deletes the UAS elements required for expression of the adjacent GAL3 gene, whereas trp1-delta63 does not. trp1-delta1 strains are therefore gal3 whereas trp1-delta63 strains remain Gal+ .

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